The group of tetracycline antibiotics is mainly bacteriostatic, with a broad spectrum of antimicrobial activity including chlamydias, mycoplasmas, rickettsias, and spirochaetes, and aerobic and anaerobic Gram –positive and Gram-negative pathogenic bacteria, and some protozoa. Tetracyclines are taken up into sensitive bacterial cells by an active transport process. Once within the cell they bind reversibly to the 30S subunit of the ribosome, preventing the binding of amino acyl transfer RNA and inhibiting protein synthesis and hence cell growth. Although, tetracycline is also inhibited protein synthesis in mammalian cells, they are not actively taken up, permitting selective effects on the infecting organism.